Journal: Stem Cell Research & Therapy
Article Title: Local application of IGFBP5 protein enhanced periodontal tissue regeneration via increasing the migration, cell proliferation and osteo/dentinogenic differentiation of mesenchymal stem cells in an inflammatory niche
doi: 10.1186/s13287-017-0663-6
Figure Lengend Snippet: Local injection of rhIGFBP5 promoted periodontal tissue regeneration and relieved local inflammation in a swine periodontitis model. a , b A minipig model of periodontitis was established. Intraoral photographs ( a ). Three-dimensional reconstructive CT image ( b ). c , d Intraoral manifestations indicated that local injection of rhIGFBP5 alleviated local inflammation in comparison with the 0.9% NaCl group at 12 weeks after injection. e Clinical assessment of periodontal tissue regeneration. f , g Three-dimensional reconstructive CT scan exhibiting bone formation after injection at 12 weeks. h Quantitative analysis of the volume of new bone formation. i - p H&E staining exhibiting new periodontal tissue regeneration in the periodontal lesion area in the 0.9% NaCl group ( i , j , m , o ), and the rhIGFBP5 injection group ( k , l , n , p ). m – p H&E staining indicated new cementum and Sharpey’s fibers in the 0.9% NaCl group ( m , o ), and the rhIGFBP5 injection group ( n , p ). q , r Quantitative analysis of new cementum. Scale bar: 1 mm ( i , k ), 50 μm ( m , n ), 20 μm ( o , p ). CEJ cemento-enamel junction, HAB height of alveolar bone, c cementum, b bone, d dentin, NC new cementum, Blue arrow , inflammatory cells; green arrow , Sharpey’s fibers; red arrow , cementoblast; black arrow , PDLSCs. Student’s t test was implemented to test statistical significance. Error bars represent SD ( n = 6). NS no significant difference. ** P ≤ 0.01. AL attachment loss, GR gingival recession, NBV new bone volume, PD probing depth, rhIGFBP5 recombinant human insulin-like growth factor binding protein 5
Article Snippet: Bone regeneration was calculated by three-dimensional reconstructive computed tomography (CT) scan (Siemens, Berlin, Germany).
Techniques: Injection, Comparison, Computed Tomography, Staining, Recombinant, Binding Assay
